Identification Of Unknown Bacteria Using Biochemical Test

Bacteria is grown in a sugar media.

A different unknown number is given to each place bacteria are collected. This test is done to help identify proteus morganella yersinia enterocolitica and helicobacter pylori. Phenotypic identification of bacteria by biochemical tests fermentation substrate utilization etc is still relevant. To identify bacteria we must rely heavily on biochemical testing.

Iii biochemical tests the staining is followed by use of various biochemical reagents and tests to get closer to the identification of bacteria. Differential media like blood agar is useful in identifying the type of haemolysis and thus the pathogenicity of various bacteria streptococci. This is based on the following chain of logic. It produces benefits for many aspects of the research of microorganisms and helps physicians correctly treat patients.

Identification of gram positive bacteria can be achieved by carrying out various biochemical tests. Few of them are required to be carried out depending upon the bacteria. When bacteria is grown in this medium there may be the production of acids or alkali or even no change in ph. Identification of bacteria by biochemical tests.

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This is one of the widely used methods and more important. Each different species of bacterium has a different molecule of dna i e dna with a unique series of nucleotide bases. Automated methods of identification of unknown bacteria. Students then use these plates to make their own unknown plate by streaking for single colonies.

The types of biochemical reactions each organism undergoes act as a thumbprint for its identification. The identification of bacteria is a careful and systematic process that uses many different techniques to narrow down the types of bacteria that are present in an unknown bacterial culture. To make the process easy and short automatic methods have been developed. I have developed software applications that will enable users to identify the organisms based on the results of their tests.

Identification of unknown bacteria by the manual method using staining reactions motility test cultural characteristics and biochemical tests is a cumbersome lengthy and time consuming process. There are many biochemical tests available for bacterial identification.